Effects of Culture Temperature on Microtuber Formation of Aconitum Carmichaelii Debx. and Aconitine- Type Alkaloid Contents
نویسنده
چکیده
SHIPING c., SHAN S.], TANAKA H. and SHOYAMA Y. Effects of culture temperature on microtuber formation of Aconitum carmichaelii Debx. and aconitine -type alkaloid contents BIOTRONICS 27, 15-20, 1998. The clonally propagated Aconitum carmichaelii Debx. was used for the investigation of microtubering. The rooting condition of propagated shoots affected the establishment of transplantation to soil indicating the Murashige-Skoog medium supplemented with 0.5 mg/l of IAA and the cultivation at 20 e are the best. Culturing at 15°e under the dark enhanced microtubering rather than lOoe or 20oe. The temperature affected the production of aconitine-type alkaloids, demonstrating that the contents of mesaconitine and hypaconitine were higher at 20 e than at 15 and lOoe.
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